Composite

Part:BBa_K4241802:Design

Designed by: Vicera Christian Nelson Tejedor   Group: iGEM22_HKU_HongKong   (2022-10-11)


CvNDI1_CeB_TRAmy3D


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1506
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1506
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1506
    Illegal PstI site found at 55
    Illegal PstI site found at 404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1506
    Illegal PstI site found at 55
    Illegal PstI site found at 404
    Illegal NgoMIV site found at 264
    Illegal NgoMIV site found at 1086
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 849


Design Notes

coding region is optimized for microalgal expression


Source

Chlorella vulgaris, Cecropia moth, rice

References